How do you dilute camptothecin?
Camptothecin is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, camptothecin should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. Camptothecin has a solubility of approximately 0.25 mg/ml in a 1:3 solution of DMSO:PBS (pH 7.2) using this method.
How do you dissolve camptothecin?
This product is soluble in DMSO (10 mg/ml). At higher concentrations, heating is required for the product to dissolve completely (approximately 10 minutes at 95 °C), but some precipitation occurs upon cooling to room temperature. It is also soluble in 1 N NaOH (50 mg/ml).
What is the solubility of CPT?
Although CPT is barely soluble (5 μM) in phosphate-buffered saline (PBS), HCPT is slightly more soluble (29 μM). To determine the solubility enhancement, a known concentration of WP6 was stirred with excess solid drug for 3 h in NaD2PO4 buffer (10 mM, pD 7.4).
Is camptothecin DMSO soluble?
This product is soluble in DMSO (10 mg/ml). At higher concentrations, heating is required for the product to dissolve completely (approximately 10 minutes at 95 °C), but some precipitation occurs upon cooling to room temperature. It is also soluble in 1 N NaOH (50 mg/ml).
What is CPT in chemistry?
Camptothecin (CPT) is a monoterpene indole alkaloid originally isolated from the bark and stem of Camptotheca acuminata. It has a pentacyclic ring structure bearing a pyrrolo[3,4-β]-quinoline moiety.
Is camptothecin soluble in water?
Solubility: Soluble in DMSO (10mg/ml), methanol (40mg/ml), 0.1N sodium hydroxide (50mg/ml) or acetic acid; insoluble in water.
What type of compound is camptothecin?
It is a pyranoindolizinoquinoline, a tertiary alcohol, a delta-lactone and a quinoline alkaloid. Camptothecin is an alkaloid isolated from the stem wood of the Chinese tree, Camptotheca acuminata. This compound selectively inhibits the nuclear enzyme DNA topoisomerase, type I.
What is the stability of camptothecin?
In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency.
What is the significance of camptothecin?
Camptothecin, an quinolone alkaloid, is used as a chemotherapeutic agent in the treatment of leukemia (Jones et al., 1997). Camptothecin complexes with type I DNA topoisomerase inhibiting both cleavage and religation reactions of DNA replication thus arresting growth of L.
Is camptothecin water soluble?
Solubility: Soluble in DMSO (10mg/ml), methanol (40mg/ml), 0.1N sodium hydroxide (50mg/ml) or acetic acid; insoluble in water.